VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb Review

Out of the Box: VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb is a crucial component for molecular biology labs, specializing in gel electrophoresis. It allows for the precise and efficient separation of DNA or RNA fragments based on size. Its 16-tooth design enables the simultaneous preparation of a significant number of samples for analysis, streamlining workflows in research and diagnostics.

My journey to acquiring this particular comb stemmed from the need to upgrade the capabilities of our university research lab. We were previously limited by combs that could only handle a smaller number of samples per gel, leading to bottlenecks in our experimental processes. The advertised precision and compatibility with our existing VWR Midi Plus system made this comb an appealing choice.

Unboxing the comb revealed a seemingly simple, yet meticulously crafted piece of equipment. The comb felt sturdy and well-made, with a smooth finish, suggesting high-quality materials were used. The teeth were uniformly spaced and precisely aligned.

Compared to generic combs we’ve used in the past, this VWR comb felt significantly more robust. We also considered combs from Hoefer and Thermo Scientific, but ultimately chose the VWR comb for its compatibility with our existing system and promising precision. The others seemed overpriced for similar specifications.

My first impression was cautiously optimistic. While the design seemed straightforward, I was eager to see how the 1.5 mm thickness and 16-tooth configuration would translate into gel loading efficiency and resolution of our DNA samples. The real test would be in the lab.

Real-World Testing: Putting VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb to the Test

First Use Experience

The first real-world application of the VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb was during a large-scale genotyping project. We were analyzing PCR products from a population study, requiring high throughput. The comb was used to create wells in agarose gels for loading DNA samples.

Performance was excellent; the 1.5 mm thickness created wells of optimal volume for our sample concentrations. The comb inserted and removed smoothly from the gel, leaving clean, well-defined wells. There were no issues with tearing or deformation of the gel matrix, even in slightly softer agarose gels.

Using this comb significantly increased our throughput compared to previous methods. The 16-tooth design allowed us to load a large number of samples onto a single gel, effectively doubling our capacity. This reduced the time required for gel electrophoresis and downstream analysis.

No significant issues were encountered during the initial use. The comb performed as expected, providing reliable and consistent well formation. The uniform well size and spacing also contributed to more even migration of DNA fragments during electrophoresis.

Extended Use & Reliability

After several months of continuous use in our lab, the VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb has proven to be a durable and reliable tool. It has been used in numerous electrophoresis experiments, consistently delivering high-quality results. The comb still performs as effectively as it did on the first day.

There are no visible signs of wear and tear on the comb, despite frequent use and cleaning. The teeth remain perfectly aligned and the material shows no signs of degradation. The comb’s robust construction has clearly contributed to its longevity.

Maintenance is straightforward. After each use, the comb is rinsed with distilled water and occasionally cleaned with a mild detergent solution. This simple cleaning procedure keeps the comb free from contaminants and ensures optimal performance.

Compared to previous experiences with less expensive combs, the VWR comb stands out in terms of durability and precision. It outperforms generic combs in both well definition and overall lifespan, making it a worthwhile investment for any molecular biology lab. Its consistently clean wells produce high-quality resolution across multiple gels, which has decreased errors and reduced the need to repeat experiments.

Breaking Down the Features of VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb

Specifications

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb has a Description of 1.5 mm x 16-Tooth Comb. This is crucial for creating wells of a specific volume and spacing.

The Dimensions are 17.5W x 26.5L x 9H cm (6⁷/8 x 10⁷/16 x 3¹⁷/32“). These dimensions ensure compatibility with standard midi-sized electrophoresis systems.

The compatible Buffer Volume is 500 mL, optimizing the running conditions for gel electrophoresis. Proper buffer volume is critical for consistent and reproducible results.

The 1.5 mm thickness creates wells that are ideal for loading standard volumes of DNA or RNA samples, minimizing spillover and ensuring accurate quantification. The 16-tooth design maximizes sample throughput, allowing for the simultaneous analysis of a significant number of samples. The overall dimensions ensure that the comb fits properly within the electrophoresis system, creating consistent and even electric fields.

Performance & Functionality

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb performs its job exceptionally well. The comb consistently creates wells of uniform size and shape, leading to reproducible electrophoretic separations. The comb’s precise construction and high-quality materials contribute to its reliable performance.

Its strengths lie in its precision, durability, and high throughput. The well-defined wells result in sharp, well-resolved bands, which are essential for accurate DNA or RNA analysis. The 16-tooth design significantly streamlines workflows, especially in high-throughput applications.

There are no major weaknesses associated with this comb. Minor improvements could involve the addition of etched markings on the comb to indicate well positions, but this is not a critical issue. The comb meets and exceeds expectations for a high-quality electrophoresis comb.

Design & Ergonomics

The comb is constructed from a durable, chemically resistant plastic. This ensures the comb can withstand repeated use and cleaning without degradation. The weight is well-balanced.

The comb is user-friendly, with a straightforward design that requires no special training. Its smooth surface and rounded edges prevent damage to the gel during insertion and removal.

Durability & Maintenance

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb is designed to last for an extended period. With proper care and maintenance, it can withstand years of regular use.

Maintenance is easy, requiring only rinsing with distilled water and occasional cleaning with a mild detergent. The comb’s simple design makes it easy to clean and prevents the buildup of contaminants.

Accessories and Customization Options

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb does not come with any additional accessories. It is specifically designed to be used with the VWR Midi Plus Horizontal Electrophoresis System.

There are no specific customization options for this particular comb. However, users can choose from a variety of other combs with different tooth sizes and configurations to suit their specific experimental needs.

Pros and Cons of VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb

Who Should Buy VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb?

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb is perfect for molecular biology researchers, diagnostic laboratories, and educational institutions needing high-throughput gel electrophoresis. It is also ideal for scientists working with PCR products, restriction fragments, and other DNA or RNA samples that require separation by size. Any lab needing to run multiple samples at once would benefit.

This comb may not be necessary for labs that only occasionally perform gel electrophoresis or require very low throughput. A comb with fewer teeth or different well sizes may be more suitable for these applications.

A must-have accessory would be a high-quality micropipette set for accurate sample loading. Using loading dye is also critical for visualizing and tracking samples during electrophoresis.

Conclusion on VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb is a reliable and essential tool for any molecular biology lab seeking high-throughput, precise gel electrophoresis. The comb’s durable construction, precise well formation, and ease of use make it a worthwhile investment.

The price of $189.99 is justified by the comb’s performance, durability, and long-term value. It is a cost-effective solution for increasing laboratory efficiency and ensuring accurate experimental results.

I would personally recommend the VWR Midi Plus Horizontal Electrophoresis Systems E1115-16MC15 Combs 1.5 Mm x 16-Tooth Comb to any researcher or laboratory professional looking to improve their gel electrophoresis capabilities. Invest in this comb to streamline your workflows and achieve high-quality results!