VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb Review

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb: Review from Real Use

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb might not be something you’d find in a bug-out bag, but in the realm of scientific research, specifically molecular biology and biochemistry, it’s as crucial as a reliable knife is to a survivalist. As someone who’s spent over a decade navigating the ins and outs of lab equipment, I’ve seen firsthand how a seemingly small component like a comb can dramatically impact the efficiency and accuracy of electrophoresis. This review is my attempt to translate the often-opaque language of scientific equipment into something understandable, useful, and relatable.

My team needed to upgrade our gel electrophoresis setup to handle a higher throughput of samples for a large-scale DNA fragment analysis project. We needed combs that would allow us to load more samples per gel, thus reducing the number of gels we needed to run. The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb seemed like a promising solution, offering a 20-tooth configuration with a 0.75 mm thickness.

Upon opening the package, I was immediately impressed with the seemingly robust construction of the comb. The acrylic material felt sturdy, not flimsy like some of the cheaper combs we’ve used in the past. Its clear design allowed for easy visualization of the wells while loading, which is often a hassle with opaque combs.

We had been using combs from Bio-Rad and Thermo Fisher Scientific previously. I chose the VWR comb because of its specific tooth configuration and competitive pricing compared to other brands offering similar features. I hoped it would streamline our gel loading process.

My initial reaction was positive. The VWR comb felt like a solid piece of equipment. It promised to improve our throughput and the clarity of the wells suggested it would simplify the sample loading process.

Real-World Testing: Putting VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb to the Test

First Use Experience

I tested the VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs in our molecular biology lab, as part of our standard DNA fragment analysis protocol. The comb was used with the VWR Midi Plus Horizontal Electrophoresis System, which is designed to resolve close molecular weight samples. I was assessing its ability to create clean, well-defined wells for loading DNA samples.

The comb performed admirably under the standard conditions of our lab. We didn’t subject it to extreme temperatures, but it was exposed to standard electrophoresis buffers (Tris-Borate-EDTA) and DNA loading dyes. The ease of use was immediately apparent – the teeth aligned well with the gel tray, and the resulting wells were uniform.

Using the VWR comb was straightforward. The comb fit securely into the gel casting tray, and creating the wells didn’t require any special techniques or adjustments. The wells were consistent and easy to load, making the process more efficient. There weren’t any immediate issues.

Extended Use & Reliability

After using the VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs for several weeks, I observed that it maintained its shape and structural integrity. The acrylic material didn’t warp or crack, even after repeated use and cleaning. This is crucial for maintaining consistent well formation over time.

The comb showed no signs of wear and tear despite being used almost daily. We cleaned it regularly with mild detergent and water and stored it properly to prevent any damage. It has proven easy to maintain.

Compared to previous experiences with cheaper combs, the VWR comb has exceeded my expectations in terms of durability and consistency. The wells are more uniform, and the loading process is noticeably faster.

Breaking Down the Features of VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb

Specifications

• Comb Thickness: 0.75 mm. This specification determines the volume of the wells created, influencing how much sample can be loaded.
• Number of Teeth: 20-tooth comb. The high tooth count enables high-throughput electrophoresis.
• Material: Acrylic. Known for its durability and chemical resistance.
• Compatibility: Specifically designed for the VWR Midi Plus Horizontal Electrophoresis System. This ensures proper fit and alignment within the system.
• Dimensions: Part of a system with overall dimensions of 17.5W x 26.5L x 9H cm (6⁷/8 x 10⁷/16 x 3¹⁷/32“). This is the overall size of the electrophoresis system it is designed to be used with.
• Well Volume: Dependent on gel thickness, but with a 0.75 mm thickness, the wells hold a sufficient volume for most DNA and RNA samples. The precise volume is determined by the gel’s depth during casting.

These specifications are critical because they dictate the resolution and throughput of the electrophoresis process. A higher tooth count allows for more samples to be analyzed simultaneously, saving time. The 0.75 mm thickness creates wells large enough to accommodate sufficient sample volume without causing band distortion.

Performance & Functionality

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb performs its job exceptionally well. It creates uniform, well-defined wells that allow for accurate sample loading and prevent cross-contamination between lanes. This is essential for reliable results.

The primary strength is its ability to produce consistent wells, which leads to improved band resolution and data accuracy. A minor area for potential improvement might be clearer markings on the comb for easier well identification, but this is a minor issue. The comb meets our expectations.

Design & Ergonomics

The comb is made from durable acrylic, which feels solid and well-constructed. Its weight is appropriate – not too heavy to be cumbersome, but substantial enough to feel stable when placing it in the gel. The design is simple and functional.

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb is user-friendly. There is no learning curve.

Durability & Maintenance

Given its robust acrylic construction, the VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb should last for a significant amount of time with proper care. We anticipate years of reliable use.

Maintenance is straightforward. Cleaning involves simply rinsing the comb with mild detergent and water after each use.

Accessories and Customization Options

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb doesn’t come with accessories beyond what’s needed for its basic function. Customization is limited, but different tooth configurations are available.

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb is compatible with the VWR Midi Plus Horizontal Electrophoresis System. Other manufacturers’ combs may or may not fit, so it’s important to check compatibility before purchasing.

Pros and Cons of VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb

Who Should Buy VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb?

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb is perfect for molecular biology labs, biochemistry labs, and genetic research facilities that require high-throughput electrophoresis. It’s ideal for researchers analyzing DNA fragments, RNA samples, or proteins.

This product may not be suitable for labs with electrophoresis systems from other manufacturers, unless they are specifically compatible. It’s also less essential for labs that perform only occasional electrophoresis.

A gel casting stand is a must-have accessory to ensure accurate and leak-proof gel casting.

Conclusion on VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb

The VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb is a dependable and efficient tool for high-throughput electrophoresis. Its robust construction, consistent well formation, and ease of use make it a valuable addition to any molecular biology lab.

The price of $179.99 is more than justified by its performance and durability. For labs seeking to streamline their electrophoresis processes, the VWR Midi Plus Horizontal Electrophoresis Systems E1115-20-0.7 Combs 0.75 Mm x 20-Tooth Comb is an excellent investment.

I would recommend this product to any lab looking to improve their electrophoresis efficiency and data accuracy. Upgrade your electrophoresis setup today and experience the difference!